Protective effects of bone marrow mesenchymal stromal cells on spinal cord injury in rats and its mechanism
ZHANG Gui-tong
LIU Jia
ZHANG Xiao-yan
ZHANG Zhi-cheng
LI Fang
MENG Fan-xing
SUN Yi-kun
SUN Tian-sheng
Abstract：Objective To investigate the protective effects of bone marrow mesenchymal stromal cells ( BMSCs ) on spinal cord injury ( SCI ) model of rats and its potential mechanism. Methods Totally 60 adult SD rats were randomly divided into 3 groups: sham group ( n = 20 ), SCI group ( n = 20 ) and BMSCs group ( n = 20 ). Model of spinal cord injury was established by modified Allen’ s assay. From the first day after injury, motor function of the rats’ hind limb was evaluated by BBB ( Basso Beattie and Bresnahan ) scores and Catwalk gait analysis and detection at 14 d. Content of myeloperoxidase ( MPO ), malondialdehyde ( MDA ), superoxide dismutase ( SOD ) in spinal cord tissue and levels of tumor necrosis factor-α ( TNF-α ), interleukin-6 ( IL-6 ), interleukin-10 ( IL-10 ) in serum were determined by ELISA analysis. Pathological changes of spinal cord tissues were observed with HE staining. Results Compared with the model group, the content of MPO in injured spinal cord at 12h after injury in BMSCs group decreased from ( 149.7 ± 28.4 ) nmol / mg to ( 116.0 ± 29.2 ) nmol / mg, MDA levels decreased from ( 58.1 ± 3.3 ) U / g to ( 41.7 ± 2.6 ) U / g, at 6 h after injury TNF-α levels in serum decreased from ( 439.8 ± 12.8 ) pg / ml to ( 314.6 ± 17.6 ) pg / ml, IL-6 levels decreased from ( 364.7 ± 16.9 ) pg / ml to ( 232.9 ± 15.2 ) pg / ml while SOD activity increased from ( 194.6 ± 14.3) U / mg to ( 362.0 ± 13.6 ) U / mg, IL-10 levels increased from ( 291.3 ± 9.8 ) pg / ml to ( 377.9 ± 17.9 ) pg / ml ( P < 0.05 ). Compared with the SCI group, BBB scores and catwalk gait analysis improved significantly at 14 d after injury in BMSCs group ( P < 0.05 ). The pathological injury of spinal cord was relieved in BMSCs group. Conclusions BMSCs administration improves the motor function after spinal cord injury. Its potential mechanism may be the inhibition of inflammatory reaction and oxidative stress reaction.
Keywords：Mesenchymal stromal cellsSpinal cord injuriesRats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 419-424 )