Correlation between serum neuron-specific enolase (NSE) and S100B protein and the severity and prognosis of acute spinal cord injury
LU Peng
SUN Tian-sheng
ZHANG Jian-zheng
ZHANG Zhi-cheng
HOU Jing-ming
Abstract：Objective To determine the levels of neuron-specific enolase ( NSE ) and S100B protein in patients with acute spinal cord injury and observe a possible relationship between the improvements of neurological function and biomarker levels. Methods The subjects of this study included 27 patients with acute spinal cord injury, serum levels of NSE and S100B protein were measured on the day of injury and 2 to 10 days after injury once daily, and evaluated neurological function on admission and during a 6-month follow-up. According to the functional recovery of sensory and motor 6 months after the injury, 27 cases were divided into Group A ( with AIS improvement group, n = 9 ) and Group B ( without AIS improvement group, n = 18 ). Twenty-five healthy adult volunteers whose age and sex matched with the patients were chosen as Group C. Results Serum NSE and S100 calcium binding protein B ( S100B protein ) levels of the normal control group were ( 14.38 ± 1.12 ) μg / L and ( 0.89 ± 0.13 ) μg / L. In group A, serum NSE levels rose to the highest on day 2 [ ( 109.56 ± 13.24 ) μg / L, t = 34.98, P < 0.05 ] after injury, then overall declined. In group B, serum NSE levels rose to the highest on day 3 [ ( 70.48 ± 10.42 ) μg / L, t = 25.48, P < 0.05 ] after injury. Within 7 days after the injury, the serum NSE level of each monitoring point in the group A were: [ ( 28.29 ± 10.89 ) μg / L, t = 6.47, P < 0.05; ( 45.26 ± 9.21 ) μg / L, t = 16.88, P < 0.05; ( 70.48 ± 10.42 ) μg / L, t = 27.23, P < 0.05; ( 43.25 ± 8.51 ) μg / L, t = 17.02, P < 0.05; ( 40.18 ± 7.89 ) μg / L, t = 16.34, P < 0.05; ( 37.59 ± 11.56 ) μg / L, t = 10.19, P < 0.05; ( 29.25 ± 8.98 ) μg / L, t = 8.33, P < 0.05 ], and were significantly higher than the group C. Within 7 days after the injury, the serum NSE level of each monitoring point in the group B were: [ ( 46.34 ± 11.25 ) μg / L, t = 14.16, P < 0.05; ( 109.56 ± 13.24 ) μg / L, t = 35.91, P < 0.05; ( 98.37 ± 8.64 ) μg / L, t = 48.25, P <0.05; ( 90.35 ± 10.33 ) μg / L, t = 36.62, P < 0.05; ( 73.55 ± 10.45 ) μg / L, t = 28.20, P < 0.05; ( 80.34 ± 9.75 ) μg / L, t = 33.66, P < 0.05; ( 71.56 ± 11.49 ) μg / L, t = 28.42, P < 0.05 ], and were significantly higher than the group C. Within 7 days after the injury, the serum S100B protein level of each monitoring point in the group A were: [ ( 1.36 ± 0.49 ) μg / L, t = 4.46, P < 0.05; ( 2.24 ± 0.62 ) μg / L, t = 10.52, P < 0.05; ( 2.92 ± 0.54 ) μg / L, t = 17.79, P < 0.05;( 2.35 ± 0.38 ) μg / L, t = 16.82, P < 0.05; ( 2.11 ± 0.33 ) μg / L, t = 15.47, P < 0.05; ( 1.92 ± 0.39 ) μg / L, t = 11.64, P <0.05; ( 1.81 ± 0.41 ) μg / L, t = 5.43, P < 0.05 ], and were significantly higher than the group C. Within 7 days after the injury, the serum S100B protein level of each monitoring point in the group B were: [ ( 2.24 ± 0.45 ) μg / L, t = 14.28, P < 0.05; ( 3.21 ± 0.62 ) μg / L, t = 18.25, P < 0.05; ( 4.02 ± 0.51 ) μg / L, t = 29.55, P < 0.05; ( 3.76 ± 0.53 ) μg / L, t = 26.15, P < 0.05; ( 3.26 ± 0.46 ) μg / L, t = 24.58, P < 0.05; ( 3.32 ± 0.45 ) μg / L, t = 25.71, P < 0.05; ( 3.12 ± 0.47 ) μg / L, t = 22.68, P < 0.05 ], and were significantly higher than the group C. Patients in the group B had significantly higher NSE levels compared with patients in the group A [ ( 109.56 ± 13.24 ) μg / L, ( 70.48 ± 10.42 ) μg / L, t = 7.41, P < 0.05 ]. Patients in the group B had significantly higher S100B protein levels compared with patients in the group A [ ( 4.02 ± 0.51 ) μg / L, ( 2.92 ± 0.54 ) μg / L, t = 5.03, P < 0.05 ]. Serum NSE protein level were negatively correlated with the American Spinal Injury Association ( ASIA ) sensation score of patients admitted to hospital, and negatively correlated with the recovery rate of sensation, and the correlation was statistically significant ( r = -0.70, P =0.04; r = -0.86, P < 0.01 ). Serum NSE protein levels were negatively correlated with the ASIA motor score of patients admitted to hospital, and negatively correlated with the recovery rate of motor, and the correlation was statistically significant ( r = -0.59, P < 0.01; r = -0.93, P < 0.01 ). Serum S100B protein levels were negatively correlated with the ASIA sensation score of patients admitted to hospital, and negatively correlated with the recovery rate of sensation, and the correlation was statistically significant ( r = -0.72, P = 0.03; r = -0.85, P < 0.01 ). Serum S100B protein level was negatively correlated with the ASIA motor score of patients admitted to hospital, and negatively correlated with the recovery rate of motor, and the correlation was statistically significant ( r = -0.71, P < 0.01; r = -0.92, P < 0.01 ). Conclusions Measuring serum levels of NSE and S100B protein over time is useful for evaluating the severity and prognosis of acute spinal cord injury.
Keywords：Spinal cord injuriesPhosphopyruvate hydratasePrognosisS100BNeuron-specific enolase
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 405-411 )