Effects of strontium on the proliferation, ALP activity and the differentiation of MC3T3 cells

LI Zhong-hai; HAN Li-wei; ZHAO Yan-tao; YANG Xiao-xiong; ZHONG Hong-bin; WANG Fu-li; HOU Shu-xun

doi:10.3969/j.issn.2095-252X.2016.03.015

Abstract：Objective To study the effects of strontium concentration on the proliferation, ALP activity and the differentiation of MC3T3-E1 cells. Methods MC3T3-E1 cells were cultured in vitro, and added with strontium of different concentrations. We used MTT to detect the proliferation of MC3T3-E1 celsl, PNPP to detect ALP activity, and RT-PCR to detect mRNA of key proteins of osteoblast differentiation.Results When the concentration of strontium was higher than 500 μg / ml, the proliferation of osteoblasts was signiifcantly decreased, and the growth was inhibited. When the concentration of strontium was 3.91 - 250.00 μg / ml, the promoting effect reached the highest level. With the decrease of strontium concentration, the promoting effect was gradually weakened. In the observation range, the effects of strontium on the activity of ALP was obviously dependent. Strontium could signiifcantly increase the ALP activity of MC3T3-E1. When the concentration of strontium was in the range of 3.91 - 125 μg / ml, the ALP activity of osteoblasts was signiifcantly increased. The expressions of RUNX2, ALP, Col1 and mRNA gradually increased with the concentration of strontium in a certain range. The expression of mRNA reached the peak value, while the concentration reached 31.25 μg / ml.Conclusions Strontium promotes the proliferation and differentiation of osteoblasts in a dose-dependent manner. However, when the concentration of strontium exceeds the optimal concentration range, its promotion effect is decreased, and the toxicity is inhibited after a certain threshold value.