A study of PVP-I as a long term preservation agent for demineralized bone matrix
ZHAO Yan-tao
HU Xian-tong
HAN Li-wei
BAI Yu-ling
ZHONG Hong-bin
Abstract：Objective To discuss the feasibility of PVP-I being the preservation of demineralized bone matrix ( DBM ).Methods Four standard strains were used to perform the sterilization experiment in repeated 4 times. PVP-I was added to MC3T3-E1, then cell proliferation and viability were determined by CCK-8 and differentiation status by PNPP and RT-PCR. Muscle implant experiment of nude mice was performed to verify whether the activity of DBM was damaged since it was immersed in PVP-I for a long time.Results The sterilization rates of PVP-I to staphylococcus aureus, monilia albican and bacillus subtilis were all of 100%, while to Escherichia coli 88.7%. CCK-8 assay showed reduced proliferation of the MC3T3-E1 cells when the PVP-I was at a high concentration ( 0.63±0.03 ) (P<0.05 ). When the concentration of PVP-I was down-regulated, a negligible effect was observed on MC3T3-E1 growth. PVP-I signiifcantly enhanced the activity of ALP and up-regulated the expressions of COL1α, ALP and RUNX2 ( 1.25±0.15, 1.38±0.14, 1.23±0.09 ) (P<0.05 ). However, PVP-I did not affect the expression of OCN. The nude mice experiment suggested that the activity of DBM was not inlfuenced by PVP-I.Conclusions PVP-I may be applied as the preservation of DBM.
Keywords：Povidone-iodineTransplantationhomologousSterilizationMicenude
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 846-849 )