Preparation of radiopaque demineralized bone matrix by dynamic decalciifcation process
ZHONG Hong-bin
HAN Li-wei
BAI Yu-long
ZHAO Yan-tao
LI Zhong-hai
Abstract：Objective To prepare demineralized bone matrix ( DBM ) with a stable calcium content and could be displayed under X-ray by accurate control of the decalcification process.Methods Raw materials were from the donor bone stored in cryopreservation. Hydrochloric acid was used as decalciifcation solution. Soft tissue removal, degreasing, cleaning, drying, grinding and other processes were applied in the preparation of bone powder. Decalciifcation time as well as decalciifcation solution ratio were adjusted, and 0.5 mol / L hydrochloric acid dynamic decalciifcation was performed. DBM sample was obtained after cleaning, freeze drying and irradiation sterilization. The pH value, calcium content and imaging under X-ray were measured. Osteoinductivity was evaluated by implantation test in nude mice.Results The content of calcium of the DBM-15-2 h, DBM-20-2 h, DBM-25-2 h, DBM-20-0.5 h, DBM-20-1 h and DBM-20-1.5 h were 1.86±0.84, 0.60±0.22, 0.42±0.12, 6.84±0.45, 2.90±0.32 and 1.59±0.26, respectively. The brightness of the DBM-20-0.5 h sample was the highest under X-ray. The bone induction activity was seen in 4 weeks. There were no signiifcant differences in osteoinductivity in 6 samples (P>0.05 ).Conclusions A stable, radiopaque and osteoinductive DBM can be obtained when bone powder and decalciifcation solution ratio is 1 g / 20 ml ( decalciifed 0.5 h ).
Keywords：Bone matrixMineralsBone regenerationTransplantationhomologousDemineralized bone
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 836-839 )