Maslinic acid regulates lung cancer cell proliferation, migration and induction to osteoclastogenesis：an in vitro study and a mechanism research
WANG Ting
YANG Xing-hai
HAN Shuai
WEI Hai-feng
WU Zhi-peng
QIAN Ming
XIAO Jian-ru
Abstract：Objective To investigate the effects of maslinic acid ( MA ) on lung cancer cell proliferation, migration and the induction role to osteoclasstogenesis, and further explore the possible molecular mechanisms of MA in lung cancer cells.Methods Lung cancer cell lines were treated with MA. Then, the cell proliferation rates were detected by MTS assay, while the migration of the tumor cell was examined by transwell assay. The condition medium from A549 cells stimulated by MA was collected, and then was used in BMM culture to observe the osteoclastogenesis. The mRNA levels of the possible targets of MA were detected by PCR assay.Results MA showed no significant inhibition on HBE cells, however, it signiifcantly suppressed the proliferation and migration of A549, PC9 and H1299 cells with a positive correlation of the MA concentration. 20 μmol / L of MA suppressed the proliferation of A549, PC9 and H1299 cell proliferation with ratios of 29%, 58% and 22%, while the inhibition ratios of 40 μmol / L MA were 68%, 82% and 66% respectively. Furthermore, MA obviously inhibited osteoclast differentiation induced by A549 cells (P<0.001 ). In addition, It was found that MA decreased the mRNA level of BCL2, MMP2 and MMP9 which were the downstream of NF-κb pathway, while it increased the mRNA level of BAX, CASP3 and CASP8 which were the key components of the mitochondrial apoptotic pathway.Conclusions MA can signiifcantly suppress proliferation and migration of lung cancer cells, as well as the osteoclastogenesis induced by lung cancer cells. Roles of MA in lung cancer cells are possibly achieved through the NF-κb pathway and the mitochondrial apoptotic pathway.
Keywords：Maslinic acidCell MovementOsteoclastsCell differentiationLung neoplasms
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 706-712 )