Effects of microRNA-383 on biological behaviors of osteosarcom
LI Shuai
GAO Yong
WANG Kun
LIU Wei
SONG Yu
YANG Shu-hua
YANG Cao
Abstract：Objective To investigate effects of miRNA-383 on biological behaviors of osteosarcoma. Methods MiR-383 plasmid vectors were constructed and transfected into osteosarcoma cells. Flow cytometer was used to calculate the apoptotic rate. MTT Cell Proliferation Assay measured the cell proliferation rate. An analysis of cell migration and invasion was performed by Transwell Cell Migration / Invasion Matrigel Assay. Analysis of cell clonogenic ability was performed by Colony formation Assay.Results Apoptotic rate of control group, non-sense group and anti-miR-383 group: ( 8.56±1.45 ) %, ( 12.6l±1.37 ) %, ( 33.20±2.59 ) % with statistical signiifcance (P<0.05 ). Migration test of control group, non-sense group and anti-miR-383 group: ( 82±5 ), ( 68±3 ) and ( 45±7 ) with statistical signiifcance (P<0.05 ). Migration and invasion test of control group, non-sense group and anti-miR-383 group: ( 82±5 ), ( 68±3 ) and ( 45±7 ) compared with ( 35±9 ), ( 32±4 ) and ( 13±5 ) respectively, with statistical significance (P<0.05 ). The clonogenic units of 3 groups: ( 12.7±4.1 ), ( 12.3±3.6 ) and ( 6.4±2.7 ).Conclusions MiR-383 can promote osteosarcoma cells’ apoptosis, and restrain proliferation, migration and invasion, and the colony formation.
Keywords：OsteosarcomaApoptosisGene expression regulationRNAneoplasmMicroRNA-383
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 701-705 )