A potential biological therapy with interleukin-10 and transforming growth factor-β for intervertebral disc degeneration in an in vitro beagle model
LI Wei
TANG Yong
LIU Tian-yi
JIA Zhi-wei
BAI Xue-dong
RUAN Di-ke
Abstract：Objective To explore the biological function and mechanism of underlying suppression of the release of inflammatory cytokines from degenerative disc cells and to evaluate the effectiveness of interleukin ( IL )-10 and transforming growth factor ( TGF )-β in suppressing the release of inflammatory cytokines from degenerative disc cells. Methods Six 1-year-old male beagle dogs were used. A beagle model of intervertebral disc degeneration ( IDD ) was established using an anular stab. The intervertebral discs were incised when the dogs were sacriifced 6 weeks later, and nucleus pulposus ( NP ) cells were isolated and cultured in vitro. The cultured degenerative NP cells were divided into 4 groups, including untreated degenerative NP cells, degenerative NP cells treated with 20 ng/ml IL-10, degenerative NP cells treated with 20 ng/ml TGF-βand degenerative NP cells treated with 20 ng/ml IL-10 and 20 ng / ml TGF-β. An enzyme-linked immunosorbent assay ( ELISA ) and real-time polymerase chain reaction ( PCR ) were performed to determine the effects of IL-10 and TGF-βon the protein and gene expressions of inflammatory cytokines. Results Higher expressions of inflammatory cytokines were observed in untreated degenerative NP cells. IL-10 and TGF-βblocked the cascade of inflammatory cytokines produced by degenerative disc cells and limited the development of inlfammatory responses. Conclusions The expressions of IL-1βand tumor necrosis factor ( TNF )-αin degenerative disc cells can be signiifcantly and stably suppressed by IL-10 and TGF-β. Thus, IL-10 and TGF-βmay be suitable biological therapeutic agents for IDD.
Keywords：Intervertebral disc degenerationAnimal experimentationInterleukin-10Transforming growth factor beta
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 230-234 )