Expressions of H19 in osteosarcoma and its relationship with the prognosis
ZHANG Hai-jun
YUAN Zhi-guo
BAI Deng-yan
Abstract：Objective To study the speciifc expression pattern of H19 gene in osteosarcoma tissues, and to explore its role in the carcinogenesis, progression, invasion and metastasis of osteosarcoma and its relationship with the prognosis. Methods 30 patients with osteosarcoma were selected from those who were treated in our hospital from 2009 to 2010, and 30 surgical excision specimens were obtained. Patients’ information was obtained, including the gender, age, location of lesions, staging according to the Enneking system, histopathological type, with or without lung metastases and survival of patients in the postoperative follow-up. The imprinting status of H19 gene was determined by the Polymerase Chain Reaction ( PCR ), Reverse Transcription-PCR ( RT-PCR ) and restrictive endonuclease ( AluI ) digestion in 30 patients with osteosarcoma. The loss of imprinting ( LOI ) of H19 gene and its relationship with different clinicopathologic parameters of osteosarcoma were studied. Results ( 1 ) The LOI rate of H19 gene was 41.67%in osteosarcoma tissues, 16.67%in adjacent neighboring tissues and 8.33%in distant neighboring tissues. The Kappa results of correlation analysis showed that the occurrence frequencies of LOI of H19 gene among different kinds of tissues stated above were signiifcantly correlated ( P<0.05 ). ( 2 ) One-way analysis of variance ( ANOVA ) revealed that the LOI of H19 gene in osteosarcoma were related with the Ennecking staging and lung metastases or not ( P<0.05 ). However, it was irrelevant with the gender, age, location, size and pathological type of patients ( P>0.05 ). ( 3 ) Log-rank univariate survival analysis suggested that the LOI of H19 gene, tumor staging, pathological type and presence of metastases or not were related with the prognosis of patients with osteosarcoma ( P<0.05 ), but the gender, age or location of lesions were not related with the prognosis ( P>0.05 ). The survival rate of the patients with the LOI of H19 gene was signiifcantly declined, and the differences were statistically signiifcant ( P<0.05 ). Conclusions The changes of imprinting status of H19 gene in osteosarcoma mean that it plays an important role in the carcinogenesis,progression, invasion and metastasis of osteosarcoma, and is related with the poor prognosis. It may become the new target to predict the patients’ prognosis.
Keywords：Gene expression regulationneoplasticOsteosarcomaPrognosisH19 gene
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 142-146 )