Effects of methylprednisolone on the neurocyte apoptotic genes such as bcl-2 following acute spinal cord injury
ZHENG Li-heng
LIN Hong-sheng
ZHANG Guo-wei
LI Jing-cong
XIE Lin
ZHANG Da-wei
Abstract：Objectives To observe the effects of methylprednisolone ( MP ) on the neurocyte apoptotic genes such as bcl-2, bax, cytc caspass-9 following acute spinal cord injury ( SCI ) and investigate the mechanisms of spinal cord recovery.Methods All experimental rabbits were divided randomly into 3 groups: sham group ( group S, 36 rabbits ), control group ( group C, 36 rabbits ) and MP treatment group ( group T, 36 rabbits ). The spinal cord injury models were made according to Allen’s weight-drop method. 6 rabbits were selected randomly and sacriifced at 8 h, 24 h, 3 d, 7 d, 14 d and 28 d after the injury. The RTFQ- PCR test were taken according to their injured spinal sections, and the results of ΔCt were compared and analyzed.Results The results of ΔCt of gene bcl-2 in group T was lower than in group C, which is statistically signiifcant (P<0.05 ). At 3 d, 14 d and 28 d after the injury, the results of ΔCt of gene bax was higher in group C than in group T, which is statistically signiifcant (P<0.05 ). No signiifcant differences were found between group C and T at 8 h, 24 h and 28 d (P>0.05 ). Group T had better expressions in the ΔCt of gene cytc and caspass-9 than group C at all time points, which is statistically signiifcant (P<0.05 ).Conclusions MP plays a protective role in treating the acute spinal cord injury by affecting the expressions of the bcl-2, cytc and caspass-9 gene.
Keywords：Spinal cord injuriesApoptosis regulatory proteinsRecovery of functionMethyprednisolone
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 58-62 )