A preliminary study of effects of prostaglandin E2 on rotator cuff injuries
DING Shu-chen
MA Chao-ran
WANG Zhe-yang
JIANG You-quan
LI Zheng
TIAN Zhen
QIU Yu-sheng
YIN Zhan-hai
Abstract：Objective To investigate the effects of prostaglandin E2 ( PGE2 ) on rotator cuff injuries. Methods The samples were divided into 5 groups, including tear group ( n=8 ), calciifed supraspinatus tendonitis group ( n=11 ), fresh cadaver group ( n=6 ), positive control group ( n=10 ) and negative control group ( n=10 ). The samples of the former 3 groups were tissues of supraspinatus tendons. The samples of the positive control group and negative control group were synoviums got from patients with serious osteoarthritis, and in the negative control group phosphate buffered saline ( PBS ) solution was used instead of primary antibodies. Before being stained with immunohistochemistry, all samples were detected by using microwave antigen retrieval. The primary antibodies were rabbit anti-human PGE2 monoclonal antibodies from ABCAM Company, which were diluted for 100 times. All the areas of cell aggregation were manually located, and the images were collected with a photomicroscope ( ×400 ). After the images were numbered, the computer random number generation software was utilized to select 8 numbered high power ifelds ( ×400 ) randomly. When all the areas except for the cell nucleus were taken as the regions of interest, the area and absorbance were measured by the Image-Pro Plus 6.0 ( IPP6.0 ) image processing software, and the mean optical density ( MOD ) was calculated. After that the expressions of PGE2 were evaluated, and the results were studied by t-test (α=0.05 ). Results The cytoplasm and extracellular matrix in the negative control group were both stained negatively, which were the same in the fresh cadaver group. In the positive control group, the cytoplasm and matrix showed strongly and weakly positive staining respectively, which were the same in the tear group. The cytoplasm and matrix in the calciifed supraspinatus tendonitis group both showed weakly positive staining. The MOD in the tear group was 0.3158±0.0204, which was 0.0907±0.0160 in the calcified supraspinatus tendonitis group and 0.0404±0.0096 in the fresh cadaver group. The expressions of PGE2 in the tear group were signiifcantly higher than that in the fresh cadaver group, and the differences were statistically signiifcant ( P<0.001 ). The expressions of PGE2 in the calciifed supraspinatus tendonitis group were slightly higher than that in the fresh cadaver group, and the differences were statistically signiifcant ( P<0.001 ). The expressions of PGE2 in the tear group were signiifcantly higher than that in the calciifed supraspinatus tendonitis group, and the differences were statistically signiifcant ( P<0.001 ). Conclusions PGE2 is highly expressed in the supraspinatus tendons of patients with rotator cuff injuries, which means the rotator cuff injuries are connected with the high expressions of PGE2 to some extent.
Keywords：Shoulder jointTendinopathyWounds and injuriesProstaglandins
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 712-715 )