Bactericidal effects of low-temperature plasma radiofrequency ablation technique on bacteria infected wounds ;in minipigs in vivo

YANG Run-gong; ZUO Tan-tan; ZHU Jia-liang; ZHANG Yu-peng; REN Gang; HOU Shu-xun

doi:10.3969/j.issn.2095-252X.2013.12.013

Abstract：Objective To observe the bactericidal effects of low-temperature plasma technique on different bacteria infected wounds in vivo. Methods Infected wounds of full-thickness skin defects were prepared in 5 minipigs, and 36 round wounds were made on the back of each minipig using specially made puncher. The diameter of each wound was 9 mm ( the area was approximately 0.64 cm2 ), and the depth was about 0.3 cm. Each wound was inoculated with 0.05 ml staphylococcus aureus ( SA ), staphylococcus epidermidis ( SE ) and pseudomonas aeruginosa ( PA ) dilution, whose concentration was 0.5×108 CFU/ml. 60 wounds infected by each of these bacteria were divided into 10 groups randomly. There were the strength 3 and 5 s group, the strength 3 and 10 s group, the strength 3 and 15 s group, the strength 6 and 5 s group, the strength 6 and 10 s group, the strength 6 and 15 s group, the strength 9 and 5 s group, the strength 9 and 10 s group, the strength 9 and 15 s group and the control group, with 6 wounds in each group. The quantitative bacterial examination of wounds in each group was carried out, and statistical analysis was performed. Results 180 infected wounds were made at 48 hours after they were inoculated with SA, SE and PA dilution. The bacterial counts in the groups treated by plasma radiofrequency ablation technique were signiifcantly decreased when compared with that in the control group, which were 0.0034, 0.0128 and 0.0058 in the strength 3, 6 and 9 groups inoculated with SA, 0.0213, 0.0005 and 0.0012 in the strength 3, 6 and 9 groups inoculated with SE and 0.0318, 0.0085 and 0.0125 in the strength 3, 6 and 9 groups inoculated with PA, with statistically signiifcant differences ( P<0.05 ). Statistical differences also existed in each strength group for different durations of operation. The longer the duration of operation was, the higher the sterilization rate became. After the same duration of operation, there were statistically signiifcant differences among the strength 3, 6 and 9 groups. The higher the strength was, the more obvious the bactericidal effects were. There were no signiifcant differences between the strength 6 and 9 groups. Conclusions There are obvious bactericidal effects of low-temperature plasma radiofrequency ablation technique on different kinds of clinical common bacteria in vivo.