The influence of magnesium alloy with calcium phosphate coating (CaP-AZ31B) on the biological properties of mouse osteoblasts

ZHANG Tao; XIA Hong; YIN Qingshui; LI Mei; ZHANG Yu; YANG Xiaoming; LAN Guobo

doi:10.3969/j.issn.1674-666X.2016.02.006

Abstract：Objective To study the influence of magnesium alloy with calcium phosphate coating (CaP-ZA31B) on the biological properties of mouse osteoblast precursor cells (MC3T3-E1). Methods In this study, they were divided into CaP-ZA31B group and two control groups [magnesium alloy (AZ31B) group and titanium alloy group]. Adhesion rates of MC3T3-E1 cells on the surface of the materials in 3 groups 2, 6, and 24 hours after co-culture were observed; Cell morphology of MC3T3-E1 1, 3, 5, 7 days after co-culture with leach liquor of materials in 3 groups were determined by using inverted microscope, and with CCK assay and BCA assay, MC3T3-E1 proliferation activities were detected, at the same time, total protein of MC3T3-E1 was also calculated in each group. Results As time of co-culture went by, adhesion rate, proliferation viability as well as total protein of MC3T3-E1 in 3 groups all increased significantly (P <0.05). At different time-points, cell adhesion rate of MC3T3-El cell in CaP-AZ31B group > titanium alloy group > AZ31B group (P <0.05). MC3T3-E1 cell in leach liquor of material in each group showed good attachment to the wall, which most of cells presented fusiform. Cell proliferation viability in CaP-AZ31B groups was better than that in AZ31B group at different time-point (P <0.05);As for total protein of MC3T3-E1, CaP-AZ31B group > titanium alloy group >AZ31B group (P <0.05). Conclusion Compared to AZ31B or titanium alloy, CaP-AZ31B has better biocompatibility with mouse osteoblast precursor cells because the material could enhance cell adhesion and proliferation viability, and improve the expression level of intracellular total protein more significantly.