Effects of chemical groups of gold surface with self-assembled monolayers on osteosarcoma cells adhesion
DENG Yinghu
LI Lihua
LI Mei
XIA Hong
Abstract：Objective To observe the effects of chemical groups of gold surface with self-assembled monolayers (SAMs) on U2-OS sarcoma cells adhesion. Methods After immersion into thiol solutions with 1%different chemical groups for 12 h respectively, the methyl (-CH3), amino (-NH2), carboxyl (-COOH) and hydroxyl (-OH) functional groups were self-assembled on Au layers, and the surface contact angles were determined respectively to evaluate the equivalent surface densities of chemical functional groups. U2-OS cells were seeded on these substrates, and the surface of bare Au layers was as control. Cell adhesion was observed under light microscope after 3, 6, 9 hours' culture, and further examination was undertaken by scanning electron microscopy (SEM) after 6 hours' culture. Results The contact angle of-OH,-COOH,-NH2 and-CH3 surface was 9.5° ± 1.2° , 18.3° ± 3.9° , 59.7° ± 3.8° and 105° ± 9.1° respectively, which there had statistical differences among these chemical groups (P <0.05). Under light microscope, after 3 hours' culture, almost all of U2-OS cells adhered onto the surface of the gold subtrates began to present a small round shape, the cells on the-CH3 surface seemed to be very loose. Six hours later, cells volume on the surface of-NH2,-COOH,-OH and control group increased, and large oval, polygonal, spindle cells had been found, but the cells on-CH3 surface were still small round. After 9 hours' incubation, there existed significant differences of cell morphology in different groups, while cells on-CH3 surface still remained round or oval. As the culture time went by, cell number increase on-CH3 surface was the least, the differences of cell number among groups at each time point had statistical significance (P <0.05); The adhesion number of U2-OS cells followed the trend:-CH3<<-OH<-COOH≈-NH2. SEM results indicated that U2-OS cells cultured on-OH and-COOH surfaces after 6 hours' incubation often exhibited polygonal and oval morphology with bigger volume and adbundant cytoplasm, and more pseudopodia extended and partial fused with each other; Most of cells on-NH2 surface showed a spindle shape, while cells cultured on-CH3 surface were smaller and in a spherical shape. Conclusion-CH3 functional group probably inhibit the adhesion of osteosarcoma cells on the gold surface with SAMs.
Keywords：OsteosarcomaTumor cellsculturedCell adhesionChemical functional groupSelf-assembled monolayer
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 238-245 )