Biocompatibility of magnesium-aluminum alloy with hydroxyapatite coating in vitro
CHEN Xuqiong
YIN Qingshui
XIA Hong
WU Zenghui
WANG Zhiyun
AI Fuzhi
WANG Jianhua
Abstract：Objective To evaluate the biocompatibility of magnesium-aluminum alloy (AZ31B) with hydroxyapatite (HA) coating in vitro. Methods The experiment included three groups, group of HA-coated AZ31B leaching liquors (H group), positive control group (P group) and blank group (N group). L929 cells were cultured with mixtures in 3 groups for 3, 5 and 7 days, the cell toxicity was graded, and cell viability was measured by WST-1. Optical density (OD) was examined and hemolysis rate was then calculated after diluted cony blood mixed with mixtures in each group. Intradermally, locally induction and stimulation were underwent on the skin of Cavia Cobaya with mixtures in 3 groups. The condition of skin sensitization of Cavia Cobaya was observed 24, 48 and 72 h after sticking removed. Results Cell toxicity was graded as 0 grade in H group and N group after 3, 5 and 7 day's culture, while the cell toxicity of part of cells in P group was graded as 3 grade. Cell viability in H and N groups were higher than that in P group, the difference had statistical significance (P >0.05). OD in H group, P group and N groups was (0.004 ± 0.001), (0.648 ± 0.050) and (0.008 ± 0.003) respectively. The rate of haemolysis was -0.6% in H group, and no hemolysis reaction was found. The skin had no obvious changes in H group 24, 48 and 72 h after sticking removed, while moderate to severe cofluent erythema on the skin was found in P group. Conclusion Experimental results showed magnesium-aluminum alloy with HA coating has good biocompatibility in vitro.
Keywords：MegnesiumAluminumAlloysHydroxyapatitesBone substitutesBiocompatible materialsHistocompatibility testingIn vitro
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 159-165 )