The influence of magnesium alloy on the cell proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells
ZHANG Tao
WU Xiaona
YIN Qingshui
XIA Hong
HUANG Huayang
ZHANG Yu
LI Mei
LAN Guobo
Abstract：Objective To investigate the influence of magnesium alloy on the cell proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs). Methods BMSCs were extracted and identified, then cultured for 1, 3, 5, 7 d in magnesium alloy (AZ31B) leaching liquids, normal culture medium were used as control. Cell proliferation activity was detected by CCK-8 method; Human BMSCs were cultured in the extracts, followed by induction of differentiation, the expression of osteogenic differentiation-related gene (COLⅠ、ALP、OPN) were detected. pH-adjusted AZ31B group (pH-AZ31B) was added to observe the influence of pH value changes on the experimental results. Results BMSCs expressed CD34 (-), CD45(-), CD44 (+), CD73 (+), CD90 (+), CD105 (+). Cell relative growth rate in AZ31B group was lower than that of control group at 1, 3, 5, 7 d after the co-culture with alloy extracts (P <0.05), toxicity in AZ31B group was rated as 2 grade; while cell activities were good in control group as well as in pH-AZ31B group (P >0.05). No statistical difference was found between AZ31B group and control group in COLⅠ gene expression (P >0.05); ALP expression in AZ31B group was relatively low compared with those in pH-AZ31B and control groups, and there was no statistical difference between pH-AZ31B group and control group (P >0.05); OPN gene expression in AZ31B group was significantly higher than control group at 6 and 12 d after differentiation induction (P<0.05), but no statistical difference was found between control group and pH-ZA31B group (P >0.05). Conclusion Magnesium alloy AZ31B do not have adverse effects on cell proliferation and osteogenic differentiation of BMSCs, the effects may be related to the changes of pH value in leaching liquids.
Keywords：MagnesiumAlloysBiocompatible materialsBone marrow mesenchymal stem cellsCellscultruedCell proliferationOsteogenic differentionGenepH value
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 99-104 )