Preparation of rhBMP-2/chitosan/dextran sulfate composite microspheres and its biocompatibility study
XIA Yuanjun
ZHANG Ying
LI Lihua
YIN Qingshui
HUANG Xianhua
XIE Huibin
XIA Hong
Abstract：Objective To prepare recombinant human bone morphogenetic protein-2/chitosan/dextran sulfate (CS/DS/rhBMP-2) composite microspheres, and to evaluate their biocompatibility in vitro. Methods CS/DS/rhBMP-2 and blank CS/DS microspheres were prepared with ionic crosslinking method, and the morphology of CS/DS/rhBMP2 microspheres was observed by scanning electron microscope (SEM). For biocompatibility examination in vitro, they were divided into CS/DS/rhBMP-2 group, CS/DS group, rhBMP-2 group and control group, cell cytotoxicity to L929 cell line of mouse fibroblasts in all groups were detected by MTT assay. Results SEM results showed CS/DS/rhBMP2 microspheres were well balling and well distributed, showing that the composite microspheres were prepared successfully. Biocompatibility examinations revealed that there were no statistical significance of optical density (OD) among CS/DS/rhBMP-2 group and other control groups after 1, 3, 5, 7 day's co-culture with L929 cells. In CS/DS/rhBMP-2 group, CS/DS group, rhBMP-2 group and control group after 2 day's co-culture with L929, cell relative growth rate (RGR) were 102.41%, 102.85%, 99.34% and 100%, and the result indicated 0, 0, 1, 0 grade according to the toxicity classification respectively. Conclusion The prepared CS/DS/rhBMP-2 microspheres shown good dispersion and cytocompatibility in vitro, may be applied in bone tissue engineering field in the future due to its potential value.
Keywords：Bone morphogenetic proteinsChitosanDextran sulfateMicrospheresDelayed-action preparationsBiocompatible materialsCytotoxicity
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 345-349 )