The influence of magnesium alloy with calcium phosphate coating on the biological behavior of rabbit skeletal muscle cells
ZHANG Tao
YIN Qingshui
XIA Hong
HUANG Huayang
ZHANG Yu
LI Mei
YANG Xiaoming
LAN Guobo
Abstract： Objective To investigate the influence of magnesium alloy with calcium phosphate coating on the biological behavior of rabbit skeletal muscle cells. Methods Rabbit skeletal muscle cells were inoculated on titanium alloy, magnesium alloy (AZ31B) and magnesium alloy with calcium phosphate coating (CaP-AZ31B), and were cultured for 2, 6, 24 h. Cell adhesion rates were calculated, and cell adhesion morphology were observed by scanning electron microscope. In AZ31B and CaP-AZ31B leaching liquids, rabbit skeletal muscle cell were cultured for 1, 3, 5, 7 d, and cultured cells without alloy extracts were used as controls. Cell morphology was observed by inverted microscope, cell proliferation was detected by CCK-8 method, cell survival at 4 d after culture were evaluated by Calcein-AM and EthD-1 double staining, and the total cellular protein were determined in those 3 groups. Results Cell adhesion rates in 3 groups increased time-dependently (P <0.05);At 2, 6, 24 h, the differences of cell adhesion rate among 3 groups had statistically significant (P <0.05). Cell relative growth rate in AZ31B group was lower than that of control group at 1, 3, 5, 7 d after the culture with alloy extracts (P <0.05), while the rate in CaP-AZ31B group was higher than that of AZ31B group at each timepoint (P <0.05); Toxicity in AZ31B group was rated as 2 grade, and in CaP-AZ31B group rated as 1 grade; Cell activities were good in CaP-AZ31B group as well as the control group without obvious red-staining cells, while part of the cells were red-stained in AZ31B group with most of them still active; Intracellular total protein content in 3 groups increased in a time-dependent manner (P <0.05), the differences of total protein content among 3 groups at each timepoint were statistically significant (P <0.05), and the ranking of the total protein content from high to low was control group, CaP-AZ31 group, AZ31B group accordingly. Conclusion Magnesium alloy with calcium phosphate coating has a good biocompatibility to rabbit skeletal muscle cells.
Keywords：MagnesiumAlloysCoatingMuscleskeletalCellsculturedBiocompatible materialsRabbits
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 21-27 )