Extraction of Lycopene and Polysaccharides from Tomato Pomace
ZHANG Bohua
FAN Qi
ZHANG Ming
ZHENG Yingchao
WU Maoyu
WANG Chongdui
WANG Li
LI Feng
MENG Xiaofeng
Abstract：During processing,tomatoes generate a large amount of by-products,which are rich in lycopene and polysaccharides.This study used tomato peel pomace,a by-product of tomato processing,as the raw material.The extraction process of lycopene and polysaccharides was optimized by using enzyme-assisted aqueous two-phase extraction.Single-factor and orthogonal experiments were designed to determine the optimal extraction conditions.The antioxidant activities of lycopene and polysaccharides from tomato peels were also evaluated in vitro.The results showed that under optimal extraction conditions,25%ethyl acetate concentration,14%dipotassium hydrogen phosphate concentration,2 000 U enzyme activity,and pH 7,the extraction rates of lycopene and polysaccharides reached 76.10 mg/100 g and 503 mg/g,respectively.Antioxidant experiments demonstrated that the extracted lycopene and polysaccharides exhibited high DPPH,hydroxyl radical,and ABTS+radical scavenging activities.Cell assays showed that lycopene significantly reduced the LPS-induced NO production in RAW 264.7 cells,demonstrating good anti-inflammatory effects.The findings provided a new approach for the efficient extraction and utilization of functional active components from tomato residue,offering significant implications for promoting the circular use of agricultural by-products and maximizing resource utilization.
Keywords：Lycopeneenzyme-assisted aqueous two-phaseextraction processpolysaccharideantioxidant activity
Publication Date：2025-10-30
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：8( 18-25 )