Determination of Chlorogenic Acid and Galuteolin in Honeysuckle by HPLC
CAO Ning
YAN Xinhuan
LIU Xuemei
PAN Shaoxiang
TAN Mengnan
WANG Li
WANG Zhen
YU Susu
LI Zhicheng
ZHENG Xiaodong
Abstract：This paper established a reverse high performance liquid chromatography(RP-HPLC)technique for the simultaneous determination of chlorogenic acid and galuteolin in honeysuckle.The ultrasonic extraction method was used for pretreatment,and the extraction solvent,extraction temperature and flow conditions were optimized.The results showed that the chlorogenic acid and galuteolin showed a good linear relationship over 0.5-100.0 mg/mL and correlation coefficients above 0.999.Recovery rate was 96%-108%,the RSD of repeatability was 2.05%and 2.44%,respectively.The method was simple and accurate,and it could be suitable for the determination of chlorogenic acid and galuteolin in fresh honeysuckle.
Keywords：Honeysucklechlorogenic acidgaluteolinreverse high performance liquid chromatography
Publication Date：2024-07-20
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：6( 17-21,28 )