Determination of Benzoic Acid in Jujube by High Performance Liquid Chromatography
ZHENG Xiao-dong
SONG Ye
PAN Shao-xiang
MENG Xiao-meng
LIU Xue-mei
YAN Xin-huan
Abstract：In the paper, a water direct extraction combined HPLC method was developed for the determination of benzoic acid in jujube. The combination pretreatment of cut-homogenization-ultrasound was adopted. Different influencing factors such as the proportion of mobile phase were studied. Based on this, real samples determination and recovery experiments were carried out. The result showed that the the mobile phases were methanol and 0.15% ammonium acetate aqueous solution (5:95, v/v). Under these conditions, the peak shape was presented well with an appropriate retention time and rapid measurement. The recoveries were 78.8%~105%, and the RSDs were 4.87%~5.42%. The linearity is good in detection ( 2=0.9990), and LOQ ( =10) was 1.5mg/kg. This method can meet the need for the quantitative detection of benzoic acid in jujube.
Keywords：Jujubebenzoic acidhigh performance liquid chromatography (HPLC)
Publication Date：2015-01-01
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：4( 33-36 )