Screening of Lipase Producing Strain
LIU Jun-jie
LIU Qian
CHEN Xiao-ning
JIANG Jun-hui
Abstract：The main research aspects and results are as following:First, 6 strains producing lipase were isolated from 10 soil samples by enrichment culture, plate streaking and fermentation. Second, the properties of lipase were studied, after fermentation under the medium including 1.5% glucose, 0.7% (NH4)2SO4, 0.1% K2HPO4, 1.25% beef cream, 0.211%MgSO4·7H2O, 1.41%olive oil and the conditions 250r/min, 30℃and 72h. The optimum reaction temperature, pH, reaction time were 50℃, 8.5 and 30min respectively. Finally, the isolated strain was conserved and identified by using conventional methods for bacteria identification.
Keywords：Microorganismlipasescreening
Publication Date：2015-01-01
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：6( 33-38 )