Application of BIOMED-2 standardized rearrangement detection in diagnosis of lymphoproliferative diseases
MA Wenmei
WANG Xiaoshu
MA Xiaoyan
YU Jingwen
PANG Xuelian
CUI Wenli
Abstract：Objective To investigate the positive rates of clonal immunoglobulin(Ig)and T-cell receptor(TCR)gene rearrangements in lymphoproliferative disorders and their clinical significance in diagnosis.Methods A standardized BIOMED-2 multiplex PCR strategy was applied to detect clonal IGH,IGK,IGL and/or TCRB,TCRD,TCRG rearrangements in 80 cases of B-cell lymphoma,37 cases of T-cell lymphoma,and 38 cases of benign proliferative disorders.Results In mature B-cell lymphomas,the overall positive rate of clonal Ig gene rearrangement was 91.14%(72/79),with detection rates of 83.54%(66/79)for IGH,56.96%(45/79)for IGK,and 30.38%(24/79)for IGL.Combined analysis of IGH and IGK achieved a positivity rate of 89.87%(71/79).In mature T-cell lymphomas,the overall positive rate of clonal TCR gene rearrangement was 60.00%(21/35),with detection rates of 48.57%(17/35)for TCRB,14.29%(5/35)for TCRG,and 8.57%(3/35)for TCRD.Combined TCRB and TCRG clonal analysis yielded a positive rate of 57.14%(20/35).Among 117 lymphoma cases,17.95%(21/117)exhibited dual Ig/TCR clonal rearrangements.In benign proliferative disorders,the positive rates for Ig and TCR gene clonal rearrangements were 26.32%(10/38)and 28.95%(11/38),respectively.Conclusion The BIOMED-2 multiplex PCR strategy provides complementary diagnostic value for clonal Ig/TCR gene rearrangement analysis in lymphoproliferative disorders.However,comprehensive diagnosis should integrate morphological,immunohistochemical,and clinical features.
Keywords：lymphomabenign proliferative disordersIg gene rearrangementsTCR gene rearrangementsgene rearrangement
Publication Date：2026-01-28
Online Publishing Date：2026-03-31(First online date of this platform, not the publication date of the document)
Pages：7( 66-72 )