Expression of TRPC1 and SBEM in breast cancer tissues and its correlation with clinicopathological features

LUO Huiyu; WU Tao; SHI Xuan; WANG Gang; YE Ling; SUN Hongxia

doi:10.3969/j.issn.1007-8096.2026.01.009

Abstract：Objective To investigate the relationship between the expression of transient receptor potential channel 1(TRPC1)and small mammary epithelial mucin(SBEM)in breast cancer tissues and the clinicopathological characteristics of patients,and to analyze the clinical value of the combination of TRPC1 and SBEM in early diagnosis of breast cancer.Methods A total of 108 breast cancer patients who were treated in Jianyang People's Hospital from April 2020 to November 2023 were selected.Immunohistochemistry was used to evaluate the protein expression levels of TRPC1 and SBEM in normal tissues and breast cancer tissues.The mRNA expression levels of TRPC1 and SBEM was detected by quantitative reverse transcription polymerase chain reaction(qRT-PCR)in breast cancer patients and normal breast tissues.The correlation between the expression levels of TRPC1 and SBEM and the clinicopathological characteristics of patients was analyzed with Spearman test.The risk factors of breast cancer metastasis in patients with breast cancer were analyzed by logistic univariate and multivariate analysis.ROC curve was used to evaluate the clinical value of TRPC1 combined with SBEM in the early diagnosis of breast cancer.Results Immunohistochemistry showed that the expression of TRPC1 and SBEM in breast cancer tissues was significantly higher than those in normal breast tissues(χ2=49.63,χ2=44.09,P<0.001).The mRNA expression levels of TRPC1 and SBEM were not related to age,histological grade,histological type,tumor size and breast cancer type,but related to tumor stages and lymph node metastasis(P<0.001).The relative expression levels of TRPC1 and SBEM were positively correlated with the clinical stage of breast cancer(r=9404,r=0.9145,P<0.001).Logistic regression analysis showed that clinical stage of breast cancer(OR=2.514,P=0.042),TRPC1(OR=9.325,P=0.01)and SBEM(OR=1.581,P=0.029)were independent risk factors for lymph node metastasis of breast cancer.ROC curve showed that TRPC1 combined with SBEM had a good predictive value in the early diagnosis of breast cancer.Conclusion The expression of TRPC1 and SBEM is increased in breast cancer tissues,which are closely related to tumor stages and lymph node metastasis of breast cancer,and have good predictive values for early diagnosis of breast cancer..