Predictive value of Glypican-3,HepPar-1,Arginase-1 and Ki-67 expression levels in hepatocellular carcinoma tissues for pathological differentiation
ZHOU Yifan
LI Xiaojie
WANG Yue
YANG Lu
DAI Yiyan
Abstract：Objective To analyze the expression levels of Glypican-3,HepPar-1,Arginase-1,and Ki-67 in hepatocellular carcinoma(HCC)tissues,and explore the predictive value of their combined detection for the pathological differentiation of HCC.Methods The pathological tissues of 98 patients with HCC admitted to our hospital were collected from July 2023 to June 2024,and the adjacent tissues of 70 cases were randomly collected.The expression levels of Glypican-3,HepPar-1,Arginase-1,and Ki-67 were detected by immunohistochemical staining,and the associations between these expressions and clinicopathological features were analyzed.The predictive value of Glypican-3,HepPar-1,Arginase-1,Ki-67 and combined detection of the four markers on pathological differentiation of HCC was analyzed by receiver operating characteristic curve(ROC).Results The positive expression rates of Glypican-3,HepPar-1,Arginase-1,and Ki-67 in HCC cancer tissues were higher than those in adjacent tissues(P＜0.05).There was no significant correlation between the positive expression of these markers and age,gender,tumor size,or differentiation grade(P＞0.05).The positive expressions of Glypican-3,HepPar-1,Arginase-1,and Ki-67 were significantly higher in patients with poorly differentiated or undifferentiated HCC than in those with moderate-to-high differentiated HCC(P＜0.05).ROC results indicated that the area under the curve(AUC)for the combined detection of Glypican-3,HepPar-1,Arginase-1,and Ki-67 in predicting HCC pathological differentiation was 0.677,which was higher than that of individual detection(Glypican-3:0.625;HepPar-1:0.600;Arginase-1:0.542;Ki-67:0.578).The specificity of combined detection was 98.70%,which was superior to that of each individual marker.Conclusion The pathological differentiation of HCC patients is related to the expressions of Glypican-3,HepPar-1,Arginase-1,and Ki-67,and the combined detection of these four markers provides higher predictive value for identifying HCC with pathological differentiation gradeⅢ～Ⅳ than individual marker detection.
Keywords：Molecular markersPathological sectionsHepatocellular carcinomaPathological differentiationPredictive value
Publication Date：2025-12-28
Online Publishing Date：2026-01-28(First online date of this platform, not the publication date of the document)
Pages：5( 1682-1686 )