Value of cell block combined with SOX17 immunohistochemical detection in diagnosis and differential diagnosis of metastatic gynecological carcinoma
FANG Wei
WANG Di
TANG Ting
CHENG Hong
CHEN Long
WU Xianzhong
Abstract：Objective To investigate the value of cell block combined with SRY-box transcription factor 17(SOX17)immunohistochemical detection in the diagnosis and differential diagnosis of metastatic gynecological carcinoma.Methods The cytological specimens of 84 cases of metastatic gynecologic carcinoma were collected,including 31 cases of peritoneal effusion,28 cases of pleural effusion,1 case of peritoneal flush fluid and 24 cases of fine needle aspiration.Among them,27 cases were metastatic gynecologic carcinoma(16 ovarian high-grade serous carcinomas,1 ovarian low-grade serous carcinoma,3 ovarian clear cell carcinomas,1 endometrioid carcinoma,1 uterine clear cell carcinoma,2 fallopian tube high-grade serous carcinomas,2 cervical mucinous adenocarcinomas and 1 cervical squamous cell carcinoma),and 57 cases were metastatic non-gynecological carcinoma(10 renal cell carcinomas,15 papillary thyroid carcinomas,2 gastric adenocarcinomas,2 colorectal adenocarcinomas,5 breast invasive carcinomas of no special type,1 pancreatic ductal adenocarcinoma,1 hepatic cholangiocarcinoma,19 lung adenocarcinomas,1 lung squamous cell carcinoma,and 1 esophageal squamous cell carcinoma).The above specimens were prepared with cell blocks,and SOX17 and PAX-8 was immunohistochemically detected.Meanwhile,the staining intensity and positive rate of tumor cells were evaluated.Results SOX17 was strongly nuclear diffuse positive in 24 metastatic gynecological carcinomas,and negative in 1 case of metastatic cervical squamous cell carcinoma in pleural effusion and 2 cases of metastatic cervical mucinous adenocarcinoma in peritoneal effusion.SOX17 was negative in 56 cases of non-gynecological metastatic carcinoma and focally positive in 1 case of metastatic pancreatic ductal carcinoma in pleural effusion.The sensitivity and specificity of SOX17 were 88.9%and 98.2%respectively in metastatic gynecological carcinoma.Conclusion SOX17 has high sensitivity and specificity in the diagnosis of metastatic gynecologic carcinoma,and SOX17 immunohistochemical detection is valuable for the diagnosis and differential diagnosis of metastatic gynecologic carcinologic specimens.
Keywords：cytologyimmunohistochemistrymetastatic gynecologic malignanciesSOX17PAX-8
Publication Date：2025-11-28
Online Publishing Date：2025-12-04(First online date of this platform, not the publication date of the document)
Pages：5( 1410-1414 )