Differences of BRAF/TERT/KRAS/NRAS/HRAS genes in preoperative risk stratification system for thyroid follicule-derived nodules
JIANG Lichao
CHEN Huang
WU Jingpeng
NIU Yiru
HUANG Qixin
WANG Xiaoxi
ZHONG Dingrong
Abstract：Objective To investigate the differential significance of BRAF,TERT,KRAS,NRAS,and HRAS genes in the preoperative risk stratification system for thyroid follicular-derived nodules.Methods A total of 112 patients with thyroid nodules meeting the inclusion criteria were selected.All patients underwent preoperative fine-needle aspiration cytology(FNAC)and genetic testing(covering 5 gene loci:BRAF,TERT,KRAS,NRAS,and HRAS).Postoperative pathological diagnosis served as the"gold standard"to analyze the differential significance of these genes in risk stratification.Results Postoperative pathological diagnoses classified the 112 cases as benign tumors(63 cases),low-risk tumors(18 cases),and malignant tumors(31 cases).Clinicopathological information of thyroid follicular tumors across different risk levels showed no statistically significant differences in gender(P=0.05),age(P=0.712),FNAC site(P=0.200),or tumor size(P=0.615).However,cytological Bethesda classification(P=0.005),capsular invasion(P=0.001),and lymph node metastasis(P=0.001)exhibited statistically significant differences(P<0.05).Genetic testing results revealed no statistically significant differences in mutation rates of BRAF(P=0.198),KRAS(P=0.675),NRAS(P=0.209),HRAS(P=0.639),TERT(P=0.070),or RAS(P=0.058)among different risk-level groups.Conclusion In the risk stratification of thyroid follicular-derived nodules,mutations in BRAF,TERT,KRAS,NRAS,HRAS,and RAS genes do not demonstrate significant differential significance,whereas cytological Bethesda classification,capsular invasion,and lymph node metastasis show positive correlations with malignancy.
Keywords：thyroid follicular tumorgenetic testingrisk stratificationfine-needle aspiration cytology
Publication Date：2025-11-28
Online Publishing Date：2025-12-04(First online date of this platform, not the publication date of the document)
Pages：5( 1399-1403 )