Expression of miR-140-3p,miR-153-5p,and miR-181a-5p in breast cancer and their clinical significance

LIU Jun; WANG Xinli; YANG Ting

doi:10.3969/j.issn.1007-8096.2025.10.005

Abstract：Objective To investigate the expression of microRNA-140-3p(miR-140-3p),microRNA-153-5p(miR-153-5p),and microRNA-181a-5p(miR-181a-5p)in breast cancer and their clinical significance.Methods Ninety patients with breast cancer who underwent surgical treatment in Xi'an Interational Medical canter 986 Hospital from February 2019 to March 2021 were enrolled.Cancer tissue specimens and adjacent tissue specimens collected during surgery were used for the study.The expression levels of miR-140-3p,miR-153-5p,and miR-181a-5p in breast cancer tissues were measured by using qRT-PCR.The Kaplan-Meier method was used to analyze the relationship between the expression of miR-140-3p,miR-153-5p,and miR-181a-5p and the prognosis of breast cancer patients.Multivariate Cox regression was used to identify the prognostic factors for breast cancer patients.Results The expression levels of miR-140-3p,miR-153-5p,and miR-181a-5p in cancer tissues were obviously lower than those in adjacent tissues(P<0.05).The proportions of patients with low miR-140-3p expression,low miR-153-5p expression,and low miR-181a-5p expression were significantly higher in breast cancer patients with TNM stage Ⅲ～Ⅳ,poor differentiation,and lymph node metastasis than in those with TNM stage Ⅰ～Ⅱ,moderate to high differentiation,and no lymph node metastasis(P<0.05).The 3-year survival rate of patients with high miR-140-3p expression in breast cancer(75.00％)was higher than that of patients with low miR-140-3p expression(28.26％)(χ2=25.569,P<0.001).The 3-year survival rate of patients with high miR-153-5p expression in breast cancer(74.47％)was higher than that of patients with low miR-153-5p expression(25.58％)(χ2=32.783,P<0.001).The 3-year survival rate of patients with high miR-181a-5p expression in breast cancer(76.19％)was higher than that of patients with low miR-181a-5p expression(29.17％)(χ2=26.012,P<0.001).MiR-140-3p,miR-153-5p,miR-181a-5p,TNM stage,and lymph node metastasis were identified as the prognostic factors for breast cancer patients(P<0.05).Conclusion The expression levels of miR-140-3p,miR-153-5p,and miR-181a-5p are significantly downregulated in breast cancer tissues.These microRNAs are closely related to the clinicopathological parameters and prognosis of breast cancer patients,and may become prognostic factors for breast cancer patients.