Diagnostic value of three pathogenic methods in the detection of Mycobacterium tuberculosis in pathological tissues

OUYANG Xiaojuan; QU Lijuan; YE Xianzong; QI Xingfeng; XU Baoqing

doi:10.3969/j.issn.1007-8096.2025.08.014

Abstract：Objective To explore the different diagnostic values of three etiological methods of auramine O fluorescent staining,fluorescent probe PCR and Xpert MTB/RIF(mycobacterium tuberculosis/rifampicin)in the detection of Mycobacterium tuberculosis in pathological tissues.Methods A total of 1130 cases of pathological tissues suspected of Mycobacterium tuberculosis infection in 900th Hospital of PLA Joint Logistic Support Force from January 2020 to October 2022 were selected for this study.Three methods of auramine O fluorescence staining,fluorescent probe PCR and Xpert MTB/RIF were used to detect Mycobacterium tuberculosis in the pathological tissues.SPSS software was used to analyze the results of three detection methods.Results Among 1130 cases of pathological tissue,208 cases were Mycobacterium positive detected by auramine O fluorescence staining and the positive rate was 18.4％;106 cases were Mycobacterium tuberculosis positive detected by fluorescent probe PCR and the positive rate was 9.4％;221 cases were Mycobacterium tuberculosis positive detected by Xpert MTB/RIF and the positive rate was 19.6％.There was no significant difference between the detection result of auramine O fluorescence staining and that of Xpert MTB/RIF(P=0.344),and the consistence of detection result was moderate(k=0.537,P＜0.001).There was statistical difference between the detection results of fluorescent probe PCR and that of Xpert MTB/RIF(P＜0.001),the positive rate of Xpert MTB/RIF was significantly higher than that of fluorescent probe PCR,and the consistence of detection result was moderate(k=0.527,P＜0.001).Conclusion Auramine O fluorescence staining has a high positive rate but insufficient specificity.Fluorescent probe PCR has good specificity but a low positive rate.Xpert MTB/RIF has a high positive rate and good specificity.Auramine O fluorescence staining,fluorescent probe PCR and Xpert MTB/RIF all has certain diagnostic value with their advantages and disadvantages.Xpert MTB/RIF is a better detection method with a high positive rate and good specificity.