Introduction to an intraoperative frozen pathological section rack
LI Rui
LI Xiao-chen
LI Ya-hui
PIAO Ying-shi
WAN Hong-fei
Abstract：Objective To introduce the usage method of an intraoperative frozen section slide holder. Methods After frozen diagnosis, residual lymph nodes and parotid specimens were selected for frozen sectioning. During the sectioning process, the sections were adhered using the 'one section two adhesions' and 'one section three adhesions' methods. Between the adhesion intervals, the frozen section slide holder was used to fix each tissue section in batches. Hematoxylin and Eosin (HE) staining was performed, and the staining effect was observed. Results After using the frozen section slide holder, none of the tissue sections on the slides showed detachment, and the staining effects were all excellent. Conclusion The stepwise slide holder not only meets the requirement of 'multiple adhesions per section', but also enables timely fixation of the tissue sections, providing convenience and assurance for performing 'multiple adhesions per section' in frozen sectioning.
Keywords：Frozen sectionPathological microtomeHE stainingFrozen section preparationQuality control
Publication Date：2025-06-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 741-743 )