Application of β-tubulin Ⅲ and CD34 in the pathological diagnosis of early invasion in non-mucinous lung adenocarcinoma
CUI Yan-mei
WANG Xiao-jiang
ZHU Hui-fang
SONG Ke-yi
YU Lin-hao
LEI Xi
HU Dan
LIU Wei
Abstract：Objective To explore the application value of β-tubulin Ⅲ and CD34 in the early invasive pathological diagnosis of non-mucinous lung adenocarcinoma.Methods Immunohistochemistry using the EnVision method was employed to detect the expression differences of β-tubulin Ⅲ and CD34 in 51 cases of atypical adenomatous hyperplasia(AAH),134 cases of adenocarcinoma in situ(AIS),403 cases of microinvasive adenocarcinoma(MIA),and 540 cases of invasive adenocarcinoma(IA).Results β-tubulin Ⅲ was negative in normal lung tissue and precursor lesions of lung adenocarcinoma(AAH,AIS).The positive expression rates in MIA and IA were 47.1%and 77.4%,respectively.The expression difference of β-tubulin Ⅲ among the groups was statistically significant(P＜0.001).In the differential diagnosis of AAH,AIS,and MIA,the specificity of β-tubulin Ⅲ positivity for diagnosing MIA was 100%,while the sensitivity was 47.1%.CD34 showed positive staining in the capillaries and basement membrane of alveolar walls in normal lung tissue,AAH,and AIS,outlining the basic structure of the alveolar walls with intact alveolar structure.In MIA and IA,CD34 staining presented a"labyrinth"pattern in the invasive foci,and the staining around invasive glands was either discontinuous or absent.Conclusion The expression of β-tubulin Ⅲ differs significantly between non-mucinous lung adenocarcinoma and precursor lesions,and CD34 staining patterns are distinct.Combined detection of both markers can aid in the early assessment of lung adenocarcinoma invasion.
Keywords：Lung adenocarcinomaβ-tubulin ⅢCD34ImmunohistochemistryPrognosis
Publication Date：2025-03-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 281-286 )