Effect of pretreatment of liquid-based cell preservation samples on HPV results
LAI Yuan-qing
ZHENG Liang-kai
FAN Yan-feng
YANG Yi-bin
Abstract：Objective To explore the influence of liquid-based cell samples before and after pretreatment on HPV detection results and the consistency of the two detection methods.Methods A total of 253 liquid-based cell samples were selected,and the positive detection rate,16/18 detection rate,mixed type detection rate and consistency of HPV were compared before and after pretreatment.Results In 253 samples,the positive detection rate of HPV after pretreatment was significantly higher than that before pretreatment,the difference was statistically significant(P＜0.05),the detection rate of 16/18 and mixed type were higher than that before pretreatment,but the difference was not statistically significant(P＞0.05).The detection results before and after pretreatment were consistent,and Kappa value was 0.739.103 samples were detected by PCR-fluorescent probe method and PCR-reverse dot hybridization method,and the detection results before and after pretreatment showed strong consistency,and the consistency was higher after pretreatment(Kappa value 0.933＞0.922).Conclusion The pre-treatment of liquid-base cell samples can significantly improve the positive rate of HPV,and more 16/18 positive number and mixed type number can be detected.Using different detection methods,the samples after pretreatment have higher consistency and stability.
Keywords：PretreatmentHPV testingPCR-reverse dot hybridizationPCR-fluorescent probe methodCervical cancer
Publication Date：2025-02-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 162-165,170 )