Preparation of frozen section of sural nerve biopsy
DUO Jian-ying
CHEN Hai
HUANG Yue
ZHU Wen-jia
DI Li
WEN Xin-mei
XU Min
WANG Min
LU Yan
DA Yu-wei
Abstract：Objective To establish a practical method for preparing frozen sections of sural nerve biopsy tissue. Methods Using pathological plastic embedding boxes and self-made embedding auxiliary tools, we explored the freezing conditions and compared the quality of sectioning and staining to select the optimal freezing method for sural nerve biopsy tissue. Results Using the self-made embedding auxiliary tool, the nerve tissue sections were complete, with vertical cross-sections and no cracks after freezing for 30 seconds. Using the pathological plastic embedding box, the sections showed tissue fragmentation, defects, morphological changes, and inclined cross-sections. Conclusion This method is simple to operate, and the tissue structure is clear after staining. It can provide a nerve biopsy report within (0.52–2.5) hours, which is of great value for the rapid diagnosis of amyloid peripheral neuropathy and vasculitic peripheral neuropathy.
Keywords：Sural nerve biopsyFrozen sectionEmbedding toolsRapid diagnosis
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 85-87 )