A new method of making copper staining control
SHI Wei-ping
FU Guang-ming
QI Hui-yang
SHI Hai-lei
FU Hai-yang
Abstract：Objective To investigate the feasibility of using non-tissue specimens as positive controls in Ruhemann's copper staining. Methods A gel wax block was prepared using copper sulfate and gelatin, then sectioned and stained together with liver tissue specimens from patients with Wilson's disease. Results The copper sulfate-gelatin sections showed distinct staining, with a large number of brick-red copper particles observed under the microscope. Copper particle deposition was also visible in the liver tissue specimens from patients with Wilson's disease. Conclusion The sections prepared from copper sulfate-gelatin can meet the requirements for copper staining controls. They can be used as an indicator for staining when tissue specimens are difficult to obtain.
Keywords：Copper stainingGelatinCopper sulfateRuhemannPositive control
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 83-84 )