SOX4 and miR-17 in colorectal cancer and their effects on HCT-116 cells
ZHANG Gang
LIU Nan
WANG Guo-qiang
WANG Wei
WU Xue-liang
ZHENG Hai-ping
ZHANG Yu-guo
Abstract：Objective To investigate the expression of sex-determining region Y-box 4(SOX4)and miR-17 in colorectal cancer and their effects on the biological behavior of HCT-116 cells.Methods The tissue samples were collected from 27 colorectal cancer patients at Hebei North University First Affiliated Hospital and Zhangjiakou First Hospital from March 2020 to January 2022.The expression levels of SOX4 and miR-17 were detected with immunohistochemistry,and their relationship with clinical pathological characteristics was analyzed.Intervention of SOX4 and miR-17 expression in HCT-116 cells was determined with lentiviral transfection technology.The expression of SOX4 and miR-17 was also detected in each group of cells,as well as changes in cell proliferation,invasion,migration ability,cell activity,and related protein expression were evaluated.Results Compared with normal tissues,the expression level of SOX4 was elevated in colorectal cancer tissues.The expression of SOX4 and miR-17 in colorectal cancer tissues was positively correlated(r=0.724,P＜0.001).The expression of SOX4 and miR-17 was statistically significant difference with the pathological stage,lymph node metastasis,and liver metastasis(P＜0.05),but not with tumor size and differentiation degree(P＞0.05).The proliferation ability,migration ability,and cell activity of SOX4 overexpressing cells were significantly higher than those of the control group and SOX4 expression inhibition group(P＜0.05).There was a statistically significant difference in the expression of migration and proliferation related proteins between the SOX4 expression inhibition group and the control group(P＜0.05).Conclusions SOX4 is upregulated in colorectal cancer tissues,promoting proliferation,migration,and invasion of colon cancer cells.miR-17 may be a downstream regulatory target of SOX4.
Keywords：Colorectal cancerSRY-related HMG-box gene 4(SOX4)miR-17 gene clusterHuman colon cancer HCT-116 cellsProliferationInvasion
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1072-1078 )