Effect of rapid HE staining with different alcohol fixatives in rapid on-site evaluation of cytology
ZHAO Lin-lin
CAO Miao
WANG Cong
ZHANG Yi-yun
ZHAO Huan
GUO Hui-qin
ZHANG Zhi-hui
Abstract：Objective To investigate the differences in technical parameters and diagnostic efficacy of different alcohol-fixed smears subjected to rapid HE(Hematoxylin-Eosin,HE)staining compared with D-Q(Diff-Quick)staining and to evaluate the efficacy of rapid HE staining for use in Rapid On-Site Evaluation(ROSE).Methods A total of 160 cytological specimens were collected for routine smear,fixed with methanol and 95%ethanol,perform rapid HE staining and D-Q staining for on-site evaluation;the detachment rate,total staining time,and diagnostic efficacy of three groups of ROSE slides,and the differences in fixation effects were compared between different fixatives by observing under the microscope and calculating the average optical density(AOD)of cell nucleus staining.Results The AOD value of the 95%ethanol HE ROSE group was closer to classical HE and significantly higher than that of the methanol HE ROSE group,with statistical differences(P＜0.001).The conformity rates of C6 grade diagnosis in the three groups of ROSE were 89.1%,88.0%,and 78.3%,respectively.Conclusions Smear fixation with 95%ethanol and rapid HE staining can make accurate diagnoses quickly while meeting ROSE time requirements.
Keywords：FixativeRapid HE stainingCytologyRapid on-site evaluationAverage optical density
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 980-984 )