Application of CK5/6 and TTF-1 dual immunostaining combined with elastin fiber staining in the diagnosis of pleural invasion in lung cancer
WENG Xiu-qin
YANG Shi-juan
YU Lin-hao
LEI Xi
HU Dan
LIU Wei
Abstract：Objective To investigate the utility of CK5/6 and TTF-1 dual immunostaining combined with elastin fiber staining in diagnosing visceral pleural invasion in lung cancer.Methods A total of 108 lung cancer surgical specimens were subject to TTF-1 and CK5/6 dual immunostaining combined with elastic fiber staining,as well as traditional elastic fiber staining.A comparison was made between the two staining methods in determining pleural invasion.Results The combined TTF-1 and CK5/6 dual immunostaining with elastic fiber staining detected pleural invasion in 44 cases.In lung adenocarcinoma,cancer cell nuclei were stained brown-yellow(TTF-1+),while in lung squamous cell carcinoma,cancer cell cytoplasm was stained bright red(CK5/6+),and elastic fibers appeared deep purple,clearly demonstrating the relationship between tumor cells and elastic fibers.In contrast,traditional elastic fiber staining showed unclear visualization of cancer cells,detecting pleural invasion in only 40 cases.Conclusion The"triple staining"method of TTF-1 and CK5/6 dual immunostaining combined with elastic fiber staining provides a more intuitive and straightforward approach for assessing pleural invasion,thereby enhancing the detection rate of pleural invasion.
Keywords：Lung tumorsCK5/6TTF-1Elastin fibersPleural invasion
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 963-966 )