Application of centrifugal precipitation smear of liquid based thin-layer cell technology in cervical cytology preparation
ZHANG Shu-zheng
LIU Yan-qing
LU Wen-tian
YU Xian-feng
LI Hong-ting
Abstract：Objective To compare the quality of liquid based centrifugation precipitation thin-layer cytology(LCT)between the classic method and one-step method,and to evaluate their application in cervical cytology examination.Methods A total of 1 000 samples of cervical liquid based cytology examination were collected in the Department of Pathology,Nanjing kindmed Medical Laboratory Co.,Ltd.from January to February 2024,and two methods of cytologic preparation,classical method and one-step method were performed.The differences in quality,efficiency,and positive detection rate of cervical lesions between the two methods were compared.Results By using the classic method,499 satisfactory and 1 unsatisfactory smears were produced,with a satisfactory rate of 99.8%;498 satisfactory and 2 unsatisfactory smears were produced by one-step method,with a satisfaction rate of 99.6%;there was no statistically significant difference in the quality between the two methods of smear production(P＞0.05).From sample oscillation to smear completion,12 samples were prepared in one batch using the classical method,which took 36 minutes.It took a total of 1500 minutes to complete the smear production of 500 samples;A one-step method was used to produce 12 slides in one batch,taking 13 minutes.It took a total of 541 minutes to complete the production of 500 sample smears.The results showed that the efficiency of one-step smear production was 176%higher than that of the classical method.The classical method detected 32 positive cases and 468 negative cases,with a positive detection rate of 6.4%;33 cases were positive and 471 cases were negative using the one-step method,with a positive detection rate of 6.4%;there was no statistically significant difference in the positive detection rate between the two methods(P＞0.05).Conclusion The one-step LCT method for cervical cancer liquid based cytology has the same quality as the classic LCT preparation method,but is more efficient.
Keywords：Liquid based thin-layer cell technologyLCT one-step methodCentrifuge sedimentation smearOne-step liquid based cell preparation methodCervical cytology preparation
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 952-956 )