Bone marrow of essential thrombocythemia:a clinicopathological analysis of 27 cases
ZHANG Yi-qing
Abstract：Objective To explore the pathological features,immunophenotypes,diagnosis,and differential diagnosis of essential thrombocythemia.Methods A retrospective analysis was conducted on the results of bone marrow biopsy,Gomori stain,immunohistochemistry,serum EPO,and molecular detection of JAK2 V617F mutation,CALR exon 9 mutation,MPL exon 10 mutation and BCR/ABL1 fusion gene in 27 patients with essential thrombocythemia from Beijing Hightrust Diagnostics.Results There were 15 males and 12 females.Among 27 cases of bone marrow biopsy,the volume of hematopoietic tissue ranged from 35%-80%.92.6%(25/27)showed large and numerous megakaryocytes,scattered distribution,rich and mature cytoplasm,deep or excessive lobulation of nuclei,rare loose clusters,and MF-0 grade with Gomori stain.7.4%(2/27)showed extensive fibrosis in the bone marrow,with large and numerous megakaryocytes,and MF-2 grade with Gomori stain,belonging to post-ET myelofibrosis.Immunohistochemistry confirmed the distribution of granulosa,erythroid,and megakaryocyte lineages.Molecular detection showed JAK2 V617F mutation,CALR exon 9,and MPL exon 10 mutations,with 92.6%(25/27)positive mutations.Conclusion ET is prone to be confused with pre-primary myelofibrosis,chronic myelogenous leukemia,and polycythemia vera in the early stage of polyhematosis.The diagnosis should be combined with clinical manifestations,histopathological characteristics,immunohistochemistry,and related gene testing for comprehensive diagnosis.
Keywords：Essential thrombocythemiaGomori stainImmunohistochemistryMolecular detectionBone marrow biopsy
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 944-947 )