Clinicopathologic features and molecular pathological diagnosis of non-tuberculous mycobacterium pulmonary disease
DONG Yu-jie
LI Kun
LIU Zi-chen
ZHANG Li
ZHOU Li-juan
CHEN Xue-jing
SU Dan
CHE Nan-ying
Abstract：Objective To analyze the clinicopathologic features and molecular pathological diagnosis of non-tuberculous mycobacterium(NTM)pulmonary disease to improve diagnosis and differential diagnostic capability of pathologists.Methods There was a retrospective analysis of 57 patients with NTM pulmonary disease in Beiing Chest Hos pital from December 2016 to April 2022.The histological morphology was observed under light microscope.Acid-faststaining was used to detect acid-fast bacilli(AFB).Fluorescent quantitative polymerase chain reaction(FQ-PCR)was performed to detect Mycobacterium tuberculosis(MTB)DNA.Mycobacteria was identified by melting curve analysis.Results Totally 57 NTM pulmonary diseases were identified.Among them,M.intracellulare was the dominant cases,followed by M.xenopi,M.kansasii,M.avium,M.abscessus and M.chelomae.The histological features of 27 surgical specimens showed typical necrotizing granulomas,atypical necrotizing granulomas,fibrous encapsulated necrosis,bronchiectasis,and nonnecrotizing granulomas.The formation of small abscess was found in focal infection by M.abscessus.AFB was detected in 57 cases.AFB was observed in necrosis,fibrinous exudate,and cytoplasm of histiocytosis.In M.intracellulare lesions,AFB commonly occurred in clusters.Singular AFB was often seen in M.kansasii infection.In addition,MTB DNA was negative in 57 cases.Conclusion The clinicopathological features of patients with NTM lung disease are atypical and nonspecific.Molecular pathological techniques can significantly improve the rate of definitive diagnosis.
Keywords：Non-tuberculosis mycobacteriaLungClinicopathologic featuresMolecular pathological diagnosisDifferential diagnosis
Publication Date：2024-07-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 599-602 )