Application of hydroxyl acrylic acid tissue fixation in detection of acid fast bacilli and HER2 protein

CHEN Zhi-qiang; WANG Ying; MI Xian-jun; LUO Jiao-xiu; CHEN Ang; LIU Chao-fan; XU Xiu-mei; GUAN Yan-fei; DENG Wen-tong; DAI Xin-zhen; DUAN Li-feng

doi:10.3969/j.issn.1007-8096.2018.04.013

Abstract：Objective To study the practical value of the environmentally friendly fixative polyhydroxy acrylic acid in the detection of acid-fast bacilli by special staining and HER2 protein in breast cancer by immunohistochemistry.Methods 72 cases of tuberculosis specimens,6 cases of leprosy specimens and 116 cases of breast invasive ductal carcinoma specimens were collected from Zhongshan Boai Hosipital and Zhongshan People~ Hospital from January 2014 to April 2017.Two pieces of tissue specimens were obtained from the same lesion site and randomly divided into Groups A and B by lottery method.Both groups A and B were fixed with 4％ neutral buffered formaldehyde and polyglycolic acid to prepare the tissue sections (72 tuberculosis;6 leprosy and 116 breast cancer specimens).Both thc sections of tuberculosis and leprosy specimens were used with the special staining to detect the mycobacterium tuberculosis,and HER2 protein in breast cancer by immunohistochemistry.After that,SPSS20.0 was used to compare and analyze the different positive rates of acid-fast bacilli and the different rate of HER2 protein in Groups A and B.Results Under the biological microscope,Groups A and B were stained with the following results:both backgrounds were clean with light blue;the cell outlines were clear,and the nuclei were blue;The results of the HER2 immunohistochemical staining in Groups A and B were as follows:the background was clear,the positive staining was accurate (in the cell membrane),the positive intensity was reliable,the color was bright,there was no background staining,and the negative and positive control staining results were reliable.The positive rates of the acid-fast bacilli in Groups A and B were 57.7％ and 59％,respectively,and these different positive rates between the two groups were statistically significant.(x2 =0,P ＞ 0.05).Besides,the coincidence rate of the acid-fast bacilli test results was 98.7％.The positive rates of HER2 protein in Groups A and group B were 24.1％ and 25.9％,respectively.There was no significant difference in the positive rate between these two groups (x2 =0.50,P ＞ 0.05).Besides,the coincidence rate of the HER2 protein test results was 98.3％.Conclusion Environmental protection fixed solution polyhydroxy acrylic acid has the potential to replace the traditional reagent 4％ neutral buffer formaldehyde in the detection of the ac id-fast bacilli by special staining and HER2 protein in breast cancer by immunohistochemistry method,which thus has the promoting potential value for clinical application.