Relationship between circulating tumor cells and clinicopathologic features in non-metastatic non-small cell lung cancer
FAN Jun-zhen
LV Ya-li
WANG Bo
SONG Xin
ZHONG Mei
WANG Qiong
ZHU Feng-wei
LIU Xing
SHI Huai-yin
Abstract：Objective This study investigated the significance of circulating tumor cells (CTCs) in peripheral blood of patients with non-metastatic non-small cell lung cancer (NMNSCLC) and the relationship between CTCs and clinicopathologic features of NMNSCLC.Methods The experimental group including 52 patients with pathologically diagnosed non-metastatic non-small cell lung cancer patients (8 stage Ⅰ,26 stage Ⅱ and 18 stage Ⅲ of 45 adenocarcinoma and 7 squamous cell carcinomas) were recruited from our hospital.And 35 healthy donors from our hospital outpatient were selected as control group.CTCs were detected by negative enrichment and immunofluorescence in situ hybridization platform (Cyttel(C)).Correlation of NMNSCLC CTCs with clinicopathological features were analysed.Results CTCs were detected in 78.8％ (41/52) non-metastatic non-small cell lung cancer patients and not detected in 35 healthy controls.The difference between NMNSCLC patients and the control cohort was statistically significant (P ＜ 0.001).And 37.5％ of stageⅠ,81.5％ of stage Ⅱ and 94.4％ of stage ⅣV patients were found to have CTC ≥1/3.2 ml (P =0.020).Furthermore,the incidence of CTCs was different between the lymph node metastasis positive (100％) and negative groups (74.4％,P =0.170).However,there was not significant difference in CTC positive rate between the groups of T staging (77.3％ in T1 vs.76.5％ in T2,P =0.701),pathological subtype (76.2％ in adenocarcinoma vs.85.7％ in squamous cell carcinoma,P =0.358) and tumor differentiation (77.8％,78.6％ and 85.7％ in low,medium and high differentiation groups,P =0.551).Conclusion Cyttel(C) CTC detection technology,which can effectively detect peripheral CTCs in non-metastatic non-small cell lung cancer,is expected to become a practical detection technology of circulating tumor cells.CTCs are significantly associated with the pathological staging of non-metastatic non-small cell lung cancer patients,and are positively related to lymph node metastasis.But it's not found that CTCs are associated with patients' gender,age,pathological subtype,degree of differentiation and T stage.
Keywords：CTCsNon-metastatic non-small cell lung cancerClinicopathologic features
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 281-284,288 )