Expression of ZNF146 in myoepithelial cells of breast intraductal carcinoma and its clinical significance

DUAN Jing-ling; BIAN Li-hui; YANG Yang; WANG Xiao-yu; WU Zheng; ZHANG Xiao-ling; XIAO Sheng-jun

doi:10.3969/j.issn.1007-8096.2018.02.012

Abstract：Purpose To detect the expression of zinc finger protein 146 (ZNF146) in myoepithelial cells within different grades of breast intraductal carcinoma,and to explore the clinicopathological diagnosis significance.Methods 74 cases of breast specimens were collected,including 10 cases of normal breast tissue and 64 cases of breast intraductal carcinoma (including 6 cases of low grade intraductal carcinoma,32 cases of intermediated grade intraductal carcinoma and 26 cases of high grade intraductal carcinoma).ZNF146 expression in myoepithelial cells of different grades breast intraductal carcinoma were detected by immunohistochemistry using EnVision two steps method,and compared with expression of myoepithelial marker CK5/6.Results ZNF146 expression in myoepithelial cells of different grades breast intraductal cancer were various in both positive rate and staining intensity.The positive rate was 100％ (6/6) in low grade intraductal carcinoma and all 6 cases showed strong staining;The positive rate was 43.8％ (14/32) in intermediated grade intraductal carcinoma,which included moderate staining of 10 cases and weak staining of 4 cases;The positive rate was 30.8％ (8/26) in high grade intraductal carcinoma which included weak expression of all 8 cases.The differences of positive rate in myoepithelial cells among different grade breast intraductal carcinoma were statistically significant (P ＜ 0.05).Meanwhile,the positive rates of ZNF146 and CK5/6 in myoepithelial cells of breast intraductal carcinoma were 43.8％ and 92.2％respectively and the difference betweent them was statistically significant (P ＜ 0.05).Conclusion ZNF146 is one of new markers in breast myoepithelial cells with limited diagnostic value in the diagnosis of breast intraductal carcinoma,and its down-reduction may be related to the progression of breast intrductal carcinoma.