Expression of ALK fusion protein in triple negative breast carcinoma and its clinical significance
ZHANG Wei
ZHANG Fan
JIAO Nan-lin
ZHOU Xiao-jun
RAO Qiu
YU Bo
SHI Shan-shan
Abstract：Objective To investigate the ALK fusion protein expression and gene status in triple-negative breast carcinoma (TNBC).Methods The expression of ALK protein in 193 patients with TNBC and 20 cases with non-triple negative breast carcinoma (NTNBC)was detected by the Ventana immunohistochemistry (IHC) method.Then,the samples that had positive expression of protein were further examined with FISH and RT-PCR.Results There were 1.04％ (2/193) ALK fusion protein positive cases among all of TNBC,which showed diffuse cytoplasmic granular coloration.The cytoplasmic and weak flaky coloration without granular sensation occurred in the focal tumor cells of 5.70％ (11/193) TNBC.The nuclear/cytoplasmic coloration occurred in 11.40％ (22/193) TNBC.Conclusions ALK gene rearrangement is not observed in TNBC.ALK fusion protein expression and copy number gain are only found in individual patient,though the correlation of them was unclear.
Keywords：Triple-negative breast carcinomaALKFISHRT-PCR
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 930-934 )