Application of rapid direct immunohistochemistry for intraoperative diagnosis of breast papillary lesions
SONG Xin
LIU Mei
YANG Li
LI Xi-ru
ZHENG Yi-qiong
Fan Jun-zhen
Shi Huai-yin
Abstract：Objective To improve intraoperative diagnostic accuracy of breast papillary lesions and to validate use of rapid direct immunohistochemistry (IHC) for intraoperative diagnosis.Methods Rapid direct IHC stains of CK5 and SMMHC antibodies directly labeled with horseradish peroxidase were performed on frozen section tissues and the intraoperative diagnostic results on frozen section tissues with or without rapid direct IHC stains were compared with final pathology diagnoses based on formalin fixed paraffin embed tissues (FFPE).Results Total 90 cases were included in this study.Intraoperative diagnoses were as follows:69 cases of intraductal papilloma;3 cases of papilloma and carcinoma in situ (DCIS);3 cases of cancer,unclear specific type;1 case of intraductal papillary carcinoma,14 cases of deferred diagnosis.All the rapid CK5 and SMMHC direct IHC stains were finished within 10 minutes.Comparing with intraoperative diagnoses based on conventional frozen section only,the combinations of frozen section and rapid intraoperative direct IHC had significantly improved intraoperative diagnostic accuracy to 98％ (P ＜ 0.01),with zero percent false negative rate and they also significantly reduced deferred diagnosis rate to 2％ (P ＜ 0.01).Conclusions Use of rapid direct IHC can significantly improve intraoperative diagnostic accuracy;Rapid direct IHC has fulfilled fast turn-around time requirements for intraoperative diagnosis,and it is feasible to be used routinely for intraoperative diagnosis.
Keywords：Breast cancerPapillary lesionsIntraoperative frozen sectionImmunohistochemistry
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 768-773 )