Clinicopathological features of BRAF V600E mutated gastrointestinal stromal tumors
SHI Shan-shan
WANG Xuan
XIA Qiu-yuan
LU Zhen-feng
YE Sheng-bin
LI Rui
MA Heng-hui
RAO Qiu
ZHOU Xiao-jun
Abstract：Aim The aims of this study were to examine the status of the BRAF gene in gastrointestinal stromal tumors (GISTs) and to analyze the clinical meaning of these molecular features.Methods The clinical data,morphology,immunohistochemistry and molecular genetics of 10 cases of BRAF-mutationed GIST were analyzed by immunohistochemistry and Sanger sequencing.Results We identified 10 cases (3.5％) of BRAF (exon 15) mutations.The clinical results indicated that BRAF-mutated GISTs tended to occur more frequently in females and stomach (7 of 10 tumors),in older individuals (mean age,54.9 years),that these tumors exhibited low risk,recurrence rate and mortality (0％).All 10 cases exhibited spindle-cell features,and the morphology,and immunophenotype of these cases were strongly positive for CD117 and DOG-1,negative for SMA,desmin and S-100.No mutations was tested in KIT (exons 9,11,13,17) and PDGFRA (exons 12,18) genes in these BRAF-mutated GISTs.Conclusion BRAF-mutanted GIST is a kind tumour of a special molecular genetic change and a good clinical prognosis.
Keywords：BRAF geneWild-type GISTIHCSequence sanger
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 737-741 )