Relationship between BRAF gene mutation and related proteins expression in papillary thyroid carcinoma
GONG Lei
CHEN Ping
LIU Xian-jun
LIU Xue-xin
Abstract：Purpose To study the clinical significance of BRAF gene mutation and D2-40,CK19 and cyclinD1 protein expression in papillary thyroid carcinoma.Method BRAF gene mutation was detected in 63 specimens of papillary thyroid carcinoma and 34 samples of thyroid papillary hyperplasia tissues by amplification refractory mutation system.The protein expression level of D2-40,CK19 and cyclinD1 was determined in the tissues by immunohistochemical staining.Their correlation was analyzed.Result BRAF gene mutation rate and D2-40,CK19 and cyclinD1 protein positive expression rate in papillary thyroid carcinoma were significantly higher than thyroid papillary hyperplasia tissues (P ＜ 0.05).In the tissues of papillary thyroid carcinoma,BRAF gene mutation rate and D2-40 and cyclinD1 protein positive expression rate with lymph node metastasis were significandy higher than those without lymph node metastasis (P ＜ 0.05).And there was no significant difference of CK19 protein expression between the lymph node metastasis group and nonmetastasized group in papillary thyroid carcinoma (P ＞ 0.05).Conclusion The joint detection of BRAF gene mutation and D2-40,CK19 and CyclinD1 protein expression contributes to the diagnosis and the differentiation of papillary thyroid carcinoma and thyroid papillary hyperplasia.It may also function as an important indicator for prognosis prediction of papillary thyroid carcinoma.
Keywords：Amplification refractory mutation systemImmunohistochemistryPapillary thyroid carcinomaBRAF gene mutationD2-40CK19CyclinD1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 270-273 )