Mechanism of Bidouyan Granules in treating chronic rhinosinusitis based on network pharmacology and molecular docking technology

CHENG Boyang; GE Jie; YANG Zhifu; WANG Jingwen

doi:10.13276/j.issn.2097-1656.2026.07.013

Abstract：Objective To explore the pharmacodynamic components,potential action targets and mechanisms of Bidouyan Granules in the treatment of chronic rhinosinusitis(CRS)by means of network pharmacology and molecular docking technology.Methods The active ingredients and action targets of Bidouyan Granules were retrieved and screened through the TCMSP database and HERB database,and the target genes were obtained from UniProt database.The relevant targets of CRS were obtained using the GeneCards and OMIM databases.The intersection targets of the drug and the disease,that is,the potential action targets,were obtained through mapping with Venny 2.1.0.Traditional Chinese medicines,active ingredients and potential targets(intersection targets)were respectively imported into Cytoscape 3.9.1 software to construct a"traditional Chinese medicines-active ingredients-targets"diagram.The intersection genes of the drug and the disease were imported into the STRING platform and Cytoscape 3.9.1 software to construct a network diagram.GO and KEGG enrichment analyses were conducted on the intersection targets through the DAVID database.Finally,molecular docking software was used to evaluate the binding energy between the main active ingredients and the core targets.Results A total of 215 active components,1 087 potential action targets of the drug,1 159 action targets related to CRS,and 246 intersection targets of the drug and the disease were screened.The main active ingredients included quercetin,luteolin,etc.The core targets included TP53,STAT3,SRC,AKT1,PIK3R1,PIK3CA,HSP90AA1,IL6,PIK3CD,TNF,etc.The results of GO enrichment analysis included responses to lipopolysaccharide,responses to bacterial molecules,membrane regions,membrane rafts,transmembrane receptor protein tyrosine kinase activity,etc.The results of KEGG pathway enrichment analysis included the AGE-RAGE signaling pathway,the PI3K-Akt signaling pathway,the MAPK signaling pathway,etc.Molecular docking results showed that there were binding sites between the core targets and the main active ingredients,and the binding energies were less than-12.552 kJ/mol,indicating relatively stable binding.Conclusion Bidouyan Granules play a role in the treatment of CRS by promoting biological processes such as anti-inflammation,anti-apoptosis,enhancing immune response,regulating ion channels and regulating lipid metabolism in the body through multi-component,multi-target and multi-pathway mechanism.This study provides a scientific basis for the treatment of CRS with Bidouyan Granules,and lays a foundation for further research on its pharmacological mechanism.