Preparation and characterization of a rabbit anti-mouse CD300e polyclonal antibody and its tissue localization in a mouse liver regeneration model
WANG Shuai
YANG Tao
WANG Dan
LIANG Defeng
CAO Yanlong
ZHUANG Ran
YIN Jikai
Abstract：Objective To prepare and characterize a rabbit anti-mouse CD300e polyclonal antibody,and to evaluate its application value in identifying and localizing CD300e+monocytes/macrophages in a mouse liver regeneration model.Methods A recombinant protein consisting of the extracellular domain of mouse CD300e fused with an Fc tag was designed and synthesized as the immunogen.New Zealand white rabbits were immunized,followed by blood collection and serum isolation.Antibody titers were determined by indirect ELISA,and antibody validity was verified by flow cytometry.Bioinformatic analyses were performed to explore the intrahepatic distribution characteristics of CD300e+monocytes/macrophages.Flow cytometry and immunofluorescence staining were used to assess changes in the hepatic infiltration of these cells after partial hepatectomy in mice.Results The indirect ELISA titer of terminal rabbit serum exceeded 1.6 × 105.Following partial hepatectomy,an increased accumulation of CD300e+macrophages was observed in the injured liver lobes.Conclusion A rabbit anti-mouse CD300e polyclonal antibody is successfully prepared in this study.CD300e+macrophages are identified in perivascular regions of injured liver lobes in mice,providing a foundational tool for further mechanistic studies on their role in promoting liver regeneration.
Keywords：CD300emacrophagehepatectomyliver regenerationpolyclonal antibodyRNA-seqspatial transcriptomicsportal hypertension
Publication Date：2026-06-30
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：7( 805-811 )