Clinical significance of single and combined detection of neutrophil-to-lymphocyte ratio,D-dimer,carcinoembryonic antigen,carbohydrate antigen 19-9,and carbohydrate antigen 72-4 in peripheral blood of patients with colorectal cancer
CHI Yanxin
ZHU Hongwei
QI Fei
ZHAO Wei
Abstract：Objective To evaluate the clinical value of single and combined detection of neutrophil-to-lymphocyte ratio(NLR),D-dimer(DD),carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9),and carbohydrate antigen 72-4(CA72-4)in peripheral blood for the diagnosis of colorectal cancer(CRC).Methods The study was conducted at Jilin People's Hospital from January to December 2024.The observation group consisted of 102 CRC patients diagnosed by pathology during this period,while the control group included 51 healthy individuals who underwent health check-ups during the same period.Peripheral blood samples were collected to measure NLR,DD,CEA,CA19-9,and CA72-4 levels.Differences in these indicators between the two groups were compared.Positive rates were calculated for individual markers and for conventional combined detection(defined as positivity if any one of NLR,DD,CEA,or CA19-9 exceeded the conventional reference threshold).The diagnostic performance of each individual and combined indicator was assessed using receiver operating characteristic(ROC)curve analysis,and a weighted combined diagnostic model was constructed by binary logistic regression.Results The levels of NLR,DD,CEA,and CA19-9 in the control group were significantly lower than those in the observation group(all P<0.05),while the difference in CA72-4 levels between the two groups was not significant.The positive rate of conventional combined detection(any of NLR,DD,CEA,or CA19-9 positive)was 90.20%(92/102),significantly higher than that of any single marker(all P<0.05).ROC curve analysis revealed that the areas under the curve(AUCs)for NLR,DD,CEA,and CA19-9 alone were 0.827,0.750,0.843,and 0.630,respectively.The weighted combined diagnostic model achieved an AUC of 0.924[(95%CI(0.883,0.966)],with a sensitivity of 87.3%and a specificity of 84.3%.Its diagnostic performance was significantly superior to that of any single marker(all P<0.01).Conclusion NLR,DD,CEA,and CA19-9 are effective auxiliary diagnostic indicators for CRC.The weighted combined diagnostic model based on binary logistic regression demonstrates high diagnostic accuracy and may serve as a useful tool in clinical auxiliary diagnosis.CA72-4 showed limited diagnostic value in this cohort.
Keywords：colorectal neoplasmscarcinoembryonic antigencarbohydrate antigen 19-9neutrophil-to-lymphocyte ratioD-dimercarbohydrate antigen 72-4combined detectiondiagnostic performance
Publication Date：2026-04-30
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：5( 589-593 )